作者
Elaine da Silva Siqueira, Vírgínia Márcia Concato, Fernanda Tomiotto-Pellissier, Taylon Felipe Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Idessania Nazareth Costa, Waldiceu Aparecido Verri Junior, Wander Rogério Pavanelli, Carolina Panis, Mário Sérgio Mantovani, Milena Menegazzo Miranda-Sapla, Ivete Conchon-Costa
发表日期
2021/1/1
期刊
Phytomedicine
卷号
80
页码范围
153373
出版商
Urban & Fischer
简介
Background
Hepatocellular Carcinoma (HCC) is extremely aggressive and presents low rates of response to the available chemotherapeutic agents. Many studies have focused on the search for alternative low-cost natural compounds with antiproliferative potential that selectively respond to liver cancer cells.
Purpose
This study assessed the in vitro direct action of trans-chalcone (TC) on cells of the human HCC HuH7.5 cell line.
Methods
We subjected the HuH7.5 tumor cells to TC treatment at increasing concentrations (12.5–100 µM) for 24 and 48 h. Cell viability was verified through MTT and 50% inhibitory concentration of cells (IC50 23.66 µM) was determined within 48 h. We quantified trypan blue proliferation and light microscopy, ROS production, mitochondrial depolarization and autophagy, cell cycle analysis, and apoptosis using Muse® cell analyzer and immunocytochemical markings of p53 and β-catenin …
学术搜索中的文章
E da Silva Siqueira, VM Concato, F Tomiotto-Pellissier… - Phytomedicine, 2021