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Vehicles are burning fuels inefficiently. Nowadays, there is a strong correlation between CO₂ emission and climate change. The article estimates a theoretical environmental impact of biofuel mixtures on the transport sector. A high ratio of road transportation in CO₂ emission caused by humanity made necessary to research the estimation of the environmental impact of biofuel mixtures. We have to clarify the emission of the transport sector in order to get information about the role of the impact that is a further step to a sustainable society. Sustainable development is a kind of development where the pace of technical development, the satiation of increasing supply and the raw materials and resources of the Earth are poised so that the rate of living and the opportunities of the future generations should not decline. One of the major goals of transport policy of the European Union is sustainable mobility. For this reason …