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The present investigation has been undertaken to establish an efficient plant regeneration and genetic transformation in safflower for the Indian cultivars. In the first phase, we studied five genotypes with respect to regeneration frequency with different hormonal concentrations. Based on these studies, HUS-305 genotype that gave high regeneration with TDZ 1.0+NAA 0.2 mg/l when compared with other genotypes was selected for further experiments. In the second phase we standardized different parameters like bacterial culture O.D, vir gene inducers, vacuum infiltration, etc influencing the transformation frequency. Also, we studied different transformation methods for adoptability in safflower and kill curves of different selection agents for the cv HUS-305. Among all the parameters studied, bacterial concentration of 0.1O.D and vacuum infiltration for 30 minutes were identified as critical for getting good …