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Dear Editor, The present study reports an outbreak of conjunctivitis due to human adenovirus serotype 8 (HAdV-8) for the first time from India. HAdV-8, was detected in 16 samples by Multiplex PCR. Eleven strains were sequenced, analysed by phylogenetic tree with pre-epidemic and global isolates and revealed no variation with pre-epidemic strains. Acute viral conjunctivitis is the most common cause of infectious conjunctivitis. It can be caused by several viruses such as Human adenoviruses (HAdV), Herpes virus, Enterovirus 70 and Coxsackie virus A24 [1]. Adenovirus is the most common agent of viral conjunctivitis representing 15%-70% of all cases of conjunctivitis worldwide [2]. HAdV (Adenoviridae family) is divided into 7 species (A to G) and 68 serotypes [3]. Serotypes 3, 4, 7, 8, 19 and 37 are responsible for 89% of the cases of viral conjunctivitis worldwide [4]. Adenoviruses are non-enveloped and show resistance to both physical and chemical agents of disinfection. Their stability against such agents make them more infectious and facilitates their transmission in the community through fomites, ophthalmic instruments and even through ocular drops [5]. Information on the monthly incidence of adenoviral conjunctivitis is essential to prevent further infection [6]. In the month of October and November 2014, an outbreak of acute viral conjunctivitis occurred in Puducherry, India, during the rainy season. In this context, the aim of the present study was: to detect adenovirus by (i) viral culture,(ii) immunofluorescence assay (IFA) and (iii) multiplex polymerase chain reaction (PCR). We also conducted a molecular epidemiological study on …