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Despite the conjunctival vaccination program against Brucellosis in Palestine for more than 15 years, brucellosis continues to be a big problem concern, with increased incidence widely noted. Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. Recent reports showed that certain cell envelope associated genes of the B. melitensis is associated with its environmental stress adaptation and intracellular survival. Identification and characterization of these genes are essential to implementing efficient disease control and prevention systems. The aim of this study is to identify the cell envelope virulence associated genes of B. melitensis strains in Palestine. A total of 80 non-repetitive B. melitensis isolates were obtained from milk samples collected from infected small ruminants in the northern districts of the West Bank, Palestine during 2013 and 2015. The milk were cultured on …