作者
Noha Mousaad Elemam, Radwa Yehia Mekky, Nada Magdy El-Ekiaby, Shereen Ahmed El Sobky, Mohammad Ahmad Mohey El Din, Gamal Esmat, Ahmed Ihab Abdelaziz
发表日期
2015/9/1
期刊
Human Immunology
卷号
76
期号
9
页码范围
687-694
出版商
Elsevier
简介
Objectives
Natural killer cells are immune safeguards against HCV infection. PU.1 is a pivotal transcription factor in the development of NK cells. This study aimed at studying the regulatory effect of miRNAs on both development and function of NK cells isolated from HCV patients.
Methods
NK cells were isolated from 17 chronic HCV patients and 12 healthy controls; after which miRNA and mRNA were quantified using qRT-PCR. Manipulating miRNA expression using mimics and antagomirs, was performed followed by investigating downstream targets as well as viral abundance.
Results
PU.1 expression levels were upregulated in NK cells of HCV patients. In silico analysis revealed PU.1 to be a potential downstream target of miR-29a∗, where miR-29a∗ overexpression in NK cells caused a significant downregulation in PU.1 mRNA. Forcing miR-29a∗ caused a downregulation of the cytotoxicity determinant NK …
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NM Elemam, RY Mekky, NM El-Ekiaby, SA El Sobky… - Human Immunology, 2015