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Microfluidic paper-based analytical devices (μPADs) are a clean-room free, cost-effective, and self-pumping flow-based rapid prototyping technique, which is compatible with a varying range of fluids. Until now, μPADs have been primarily fabricated using crayons, and plotting-machine and solid-ink printers. These devices can be easily employed for various detection schemes, such as electrical, electrochemical, and optical. The presence of a capillary effect in the chromatograph paper has made μPADs independent of a passive device, such as pumps and valves. In this paper, an alternative and a novel method is proposed to achieve the μPADs effortlessly using a 3-D printer (3DP), which has many advantages over the existing methods. For creating hydrophobic barriers for microchannel walls, polycaprolactone (PCL) filament was used with fused-deposition modeling (FDM) 3DP. PCL filaments were deposited on …