查看文章

The dependence of induced myelomonocytic cell differentiation, and regulation of the RB tumor suppressor gene during this process, on the c‐fms gene product, the CSF‐1 lymphokine receptor, was determined in HL‐60 promyelocytic leukemia cells. Adding a monoclonal antibody with specificity for the c‐fms gene product to cells treated with various inducers of myelomonocytic or macrophage differentiation, including retinoic acid and 1,25‐dihydroxy vitamin D3, inhibited the rate of differentiation. During the period of inducer treatment usually preceding onset of differentiation, longer periods of antibody exposure caused greater inhibition of differentiation. In a stable HL‐60 transfectant overexpressing the CSF‐1 receptor at the cell surface due to a constitutively driven c‐fms trans gene, the rate of differentiation was enhanced compared to the wild type cell, consistent with a positive regulatory role for the CSF‐1 …