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Retinoic acid (RA), bromodeoxyuridine (BrdU), and the Δ205 mutant polyoma middle T antigen affect the expression of a common ensemble of proteins in HL-60 human myeloblastic leukemia cells. Each of these agents is known to be able to prime HL-60 cells and accelerate subsequently induced myeloid or monocytic differentiation and G0 cell cycle arrest, suggesting that they have equal or identical cellular targets relevent to the early stages of inducing cell differentiation and G0 arrest. As a test of this possibility, a survey of protein expression changes induced by RA, BrdU, or Δ205 transfection was performed. Retinoic acid induced numerous changes within h. Bromodeoxyuridine caused larger numbers of changes, whereas Δ205 caused a more limited number. Among the hundreds of affected proteins detected, there were comparable numbers of up-or downregulated proteins. A small number changed …