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To assess the reliability of automated measurements of the total antral follicle count (AFC) made using Sono‐Automatic Volume Count (SonoAVC), and to compare these to two‐dimensional (2D) and manual three‐dimensional (3D) techniques.

Fifty‐five subjects aged under 40 years who had 3D transvaginal ultrasound examination in the early follicular phase of their menstrual cycle were prospectively recruited. 3D datasets were acquired and subsequently analyzed. The total AFC (2–10 mm antral follicles) was calculated by two observers using three independent methods: 2D real‐time equivalent (2D‐RTE), 3D manual multiplanar view (3D‐MPV), and SonoAVC. For measurements made using SonoAVC, the initial automated count (sAVC‐AA) was recorded and postprocessing (sAVC‐PP) then applied to identify follicles that had been missed or incorrectly included. Intraclass correlation and …