作者
Ali Flores-Pérez, Laurence A Marchat, Sergio Rodríguez-Cuevas, Verónica Piña Bautista, Lizeth Fuentes-Mera, Diana Romero-Zamora, Anabel Maciel-Dominguez, Olga Hernández de la Cruz, Miguel Fonseca-Sánchez, Erika Ruíz-García, Horacio Astudillo-de la Vega, César López-Camarillo
发表日期
2016/12
期刊
BMC cancer
卷号
16
页码范围
1-12
出版商
BioMed Central
简介
Background
Aberrant expression of microRNAs has been associated with migration of tumor cells. In this study, we aimed to investigate the biological significance of miR-944 whose function is unknown in breast cancer.
Methods
MiR-944 expression in breast cancer cells and tumors was evaluated by Taqman qRT-PCR assays. Transcriptional profiling of MDA-MB-231 cells expressing miR-944 was performed using DNA microarrays. Cell viability, migration and invasion were assessed by MTT, scratch/wound-healing and transwell chamber assays, respectively. The luciferase reporter assay was used to evaluate targeting of SIAH1, PTP4A1 and PRKCA genes by miR-944. SIAH1 protein levels were measured by Western blot. Silencing of SIAH1 gene was performed by RNA interference using shRNAs.
Results
Our data …
学术搜索中的文章
A Flores-Pérez, LA Marchat, S Rodríguez-Cuevas… - BMC cancer, 2016