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Oxygen transfer in submerged cultures of basidiomycetes is a key factor for efficient fungal growth. However, conventional stirred tank and airlift reactors are not utterly suitable for basidiomycetes culturing because they promote high shear stress to the mycelial hyphae and favor rapid agglomeration of mycelial pellets, compromising the diffusion of oxygen to the inner side of mycelium. We describe an original reactor design that overcome some of the limitations of conventional bioreactors used in submerged cultures of basidiomycetes. The strategy was to use a mechanism that permits simultaneous axis rotation and air injection, being the agitation promoted by an L-shaped tube. Adherence of mycelium and shear stress is avoided once only rounded surfaces exist inside the bioreactor. Ceriporiopsis subvermispora was selected as a model basidiomycete. The most productive system employed sucrose/corn steep …