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Little information is available in the literature concerning the kinetics of the production of exopolysaccharides (EPS). The kinetics of growth, substrate consumption and lasiodiplodan (LAS) production by the filamentous fungus Lasiodiplodia theobromae MMPI were studied in a new bioreactor design (Low-Shear Aerated and Agitated Bioreactor; LSAAB) using media based on glucose and glycerol. The kinetic parameters of cultivation were compared with fermentations conducted in a stirred tank bioreactor (STR) and in shake flasks. Cultivation in LSAAB using glucose resulted in 3.17 ± 0.16 g.L⁻¹ of LAS, a value lower than that obtained in shake-flasks (6.49 ± 0.03 g.L⁻¹), but somewhat higher than obtained in STR (0.70 ± 0.12 g.L⁻¹). When glycerol was used as substrate, the production of LAS was less effective than glucose. From the fungal biomass produced, as well as the related growth kinetic …