作者
Reem T Atawia, Robert K Batori, Coleton R Jordan, Simone Kennard, Galina Antonova, Thiago Bruder-Nascimento, Vinay Mehta, Muhammad I Saeed, Vijay S Patel, Tohru Fukai, Masuko Ushio-Fukai, Yuqing Huo, David JR Fulton, Eric J Belin de Chantemèle
发表日期
2023/10
期刊
Hypertension
卷号
80
期号
10
页码范围
2059-2071
出版商
Lippincott Williams & Wilkins
简介
BACKGROUND
Type 1 diabetes (T1D) is a major cause of endothelial dysfunction. Although cellular bioenergetics has been identified as a new regulator of vascular function, whether glycolysis, the primary bioenergetic pathway in endothelial cells (EC), regulates vascular tone and contributes to impaired endothelium-dependent relaxation (EDR) in T1D remains unknown.
METHODS
Experiments were conducted in Akita mice with intact or selective deficiency in EC PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3), the main regulator of glycolysis. Seahorse analyzer and myography were employed to measure glycolysis and mitochondrial respiration, and EDR, respectively, in aortic explants. EC PFKFB3 (Ad-PFKFB3) and glycolysis (Ad-GlycoHi) were increased in situ via adenoviral transduction.
RESULTS
T1D increased EC glycolysis and elevated EC expression of PFKFB3 and NADPH …
学术搜索中的文章
RT Atawia, RK Batori, CR Jordan, S Kennard… - Hypertension, 2023