作者
Joy S Xiang, Jasmine R Mueller, En-Ching Luo, Brian A Yee, Danielle Schafer, Jonathan C Schmok, Frederick E Tan, Katherine Rothamel, Rachael N McVicar, Elizabeth M Kwong, Ben A Croker, Krysten L Jones, Hsuan-Lin Her, Chun-Yuan Chen, Anthony Q Vu, Wenhao Jin, Samuel S Park, Phuong Le, Kristopher W Brannan, Eric R Kofman, Yanhua Li, Alexandra T Tankka, Kevin D Dong, Yan Song, Alex E Clark, Aaron F Carlin, Eric L Van Nostrand, Sandra L Leibel, Gene W Yeo
发表日期
2022/2/23
期刊
Research Square
出版商
American Journal Experts
简介
The COVID-19 pandemic is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The betacoronvirus has a positive sense RNA genome which encodes for several RNA binding proteins. Here, we use enhanced crosslinking and immunoprecipitation to investigate SARS-CoV-2 protein interactions with viral and host RNAs in authentic virus-infected cells. SARS-CoV-2 proteins, NSP8, NSP12, and nucleocapsid display distinct preferences to specific regions in the RNA viral genome, providing evidence for their shared and separate roles in replication, transcription, and viral packaging. SARS-CoV-2 proteins expressed in human lung epithelial cells bind to 4773 unique host coding RNAs. Nine SARS-CoV-2 proteins upregulate target gene expression, including NSP12 and ORF9c, whose RNA substrates are associated with pathways in protein N-linked glycosylation ER processing and …
学术搜索中的文章
JS Xiang, JR Mueller, EC Luo, BA Yee, D Schafer… - Research Square, 2022