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In this paper, a low-loss, spiral lattice photonic crystal fiber (PCF)-based plasmonic biosensor is proposed for its application in detecting various biomolecules (i.e., sugar, protein, DNA, and mRNA) and biochemicals (i.e., serum and urine). Plasmonic material gold (Au) is employed externally to efficiently generate surface plasmon resonance (SPR) in the outer surface of the PCF. A thin layer of titanium oxide (TiO₂) is also introduced, which assists in adhering the Au layer to the silica fiber. The sensing performance is investigated using a mode solver based on the finite element method (FEM). Simulation results show a maximum wavelength sensitivity of 23,000 nm/RIU for a bio-samples refractive index (RI) detection range of 1.32–1.40. This sensor also exhibits a very low confinement loss of 0.22 and 2.87 dB/cm for the analyte at 1.32 and 1.40 RI, respectively. Because of the ultra-low propagation loss, the proposed sensor can be fabricated within several centimeters, which reduces the complexity related to splicing, and so on.