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The study reported here evaluated retinal imaging technology as a means of permanent identification of 4-H beef and sheep. The OptiReader» Device was used to capture digital images of 491 beef and 220 sheep during 4-H enrollment. A total of 317 beef and 159 sheep were re-imaged. The on-site visual verification rate was 96.2% for beef and 100% for sheep. A visual verification exercise showed that individuals could identify a pair of retinal images as a match 98.6% of the time for beef. Retinal imaging is a viable method for enrolling beef and sheep.