作者
Frederic D Sigoillot, Susan Lyman, Jeremy F Huckins, Britt Adamson, Eunah Chung, Brian Quattrochi, Randall W King
发表日期
2012/4
期刊
Nature methods
卷号
9
期号
4
页码范围
363
出版商
Nature Publishing Group
简介
Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.
学术搜索中的文章
FD Sigoillot, S Lyman, JF Huckins, B Adamson… - Nature methods, 2012