作者
Alexander Swidsinski, Axel Ladhoff, Annelie Pernthaler, Sonja Swidsinski, Vera Loening–Baucke, Marianne Ortner, Jutta Weber, Uwe Hoffmann, Stefan Schreiber, Manfred Dietel, Herbert Lochs
发表日期
2002/1/1
期刊
Gastroenterology
卷号
122
期号
1
页码范围
44-54
出版商
WB Saunders
简介
BACKGROUND & AIMS
Microorganisms that directly interact with the intestinal mucosa are obscured by fecal flora and poorly characterized.
METHODS
We investigated the mucosal flora of washed colonoscopic biopsies of 305 patients with bowel inflammation and 40 controls. The microbial cultures were validated by quantitative polymerase chain reaction with subsequent cloning and sequencing, fluorescence in-situ hybridization, and electron microscopy.
RESULTS
We found high concentrations of mucosal bacteria in patients with bowel inflammation, but not in controls. The concentrations of mucosal bacteria increased progressively with the severity of disease, both in inflamed and non-inflamed colon. In patients with >10,000 cfu/μL, a thick bacterial band was attached to the intact mucosa without signs of translocation. Patients with inflammatory bowel disease (IBD) and concentrations of mucosal bacteria >50 …
引用总数
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