查看文章

N’-[(E)-(4-Chloro-3-nitrophenyl)methylidene]pyridine-4-carbohydrazide (Hyd) was synthesized via condensation of 4-chloro-3-nitro-benzaldehyde and isonicotinohydrazide (INH) in ethanol in the presence of a few drops of acetic acid. This synthesized compound was characterized by a number of spectroscopic methods including UV, IR, NMR (¹H, ¹³C), and mass spectrometry, and by elemental analysis. Molecular structure properties of Hyd and INH were investigated using density functional theory (DFT) via B3LYP/6-31G (d,p) method, whereas the antioxidant effect was evaluated by superoxide anion scavenging assays. Results obtained from this investigation indicate high antioxidant effect of the Hyd with an IC₅₀ value of 32.37 ± 0.45 μg/mL, which is comparable with that of ascorbic acid (AA). In addition, toxicity of the synthesized compound was evaluated by in vitro and in vivo bioassays using …