作者
P Lalitha, MN Siti Suraiya, KL Lim, SY Lee, AR Nur Haslindawaty, YY Chan, Asma Ismail, Zainul F Zainuddin, M Ravichandran
发表日期
2008/9/1
期刊
Journal of Microbiological Methods
卷号
75
期号
1
页码范围
142-144
出版商
Elsevier
简介
A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/µL and 103 CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.
学术搜索中的文章
P Lalitha, MNS Suraiya, KL Lim, SY Lee… - Journal of Microbiological Methods, 2008