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The discharge of dye-contaminated wastewater into natural waterways presents a substantial risk to human and environmental health, therefore necessitating the treatment and removal of toxic dyes from colored wastewaters before their release into the ecosystem. The aim of this study was to isolate and characterize bacterial strains capable of decolorizing and/or degrading azo dyes commonly applied in textile production (monoazo dye Reactive Orange 16 and diazo dye Reactive Green 19) from activated sludge systems used in the treatment of (textile) wastewater. Following a prescreening of 125 isolates for their decolorization potential five strains were retained for further evaluation of decolorization rate and effects of physicochemical parameters using a microtiter plate method. Of those five strains, one strain belonging to the genus Acinetobacter (ST16.16/164) and another belonging to Klebsiella (ST16.16/034 …