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16S rRNA chain was cleaved by RNase H within covalent adduct with a 2', 3'-0-[4-N-(2-chloroethyl)-N-methylamino] benzylidene derivative of d (pACCTTGTT) rA. It was shown that no less than 50% of cleavage occurs at the 1498-1506 site. The selectivity of alkylation and correspondingly the cleavage by RNase H, at this site practically does not increase when RNA was alkylated to a low extent, and also when a small excess of the reagent in respect to rRNA was used.