作者
Li Chen, Qun Yue, Xinyu Zhang, Meichun Xiang, Chengshu Wang, Shaojie Li, Yongsheng Che, Francisco Javier Ortiz-López, Gerald F Bills, Xingzhong Liu, Zhiqiang An
发表日期
2013/12
期刊
BMC genomics
卷号
14
页码范围
1-18
出版商
BioMed Central
简介
Background
The antifungal therapy caspofungin is a semi-synthetic derivative of pneumocandin B0, a lipohexapeptide produced by the fungus Glarea lozoyensis, and was the first member of the echinocandin class approved for human therapy. The nonribosomal peptide synthetase (NRPS)-polyketide synthases (PKS) gene cluster responsible for pneumocandin biosynthesis from G. lozoyensis has not been elucidated to date. In this study, we report the elucidation of the pneumocandin biosynthetic gene cluster by whole genome sequencing of the G. lozoyensis wild-type strain ATCC 20868.
Results
The pneumocandin biosynthetic gene cluster contains a NRPS (GLNRPS4) and a PKS (GLPKS4) arranged in tandem, two cytochrome P450 monooxygenases, seven other modifying enzymes, and genes for L-homotyrosine biosynthesis, a component of the …
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L Chen, Q Yue, X Zhang, M Xiang, C Wang, S Li, Y Che… - BMC genomics, 2013