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Several species of bacteria can be utilized to degrade the residual insecticides. One of them was chosen from the genus Lactobacillus. The purposes of soils in Merdeka Village, Karo Regency, North Sumatra, Indonesia as well as to investigate bacteria which is capable of surviving in DDT-contaminated soil. Method for the residual analysis of the soil utilizes Quechers tube, and the bacterial propagation were performed in the medium mineral 4 (MM4). Purification to bacterial isolates was carried out in the Plate Count Agar (PCA) medium and subsequently followed by morphological identification. The results of this study show that the soil were contaminated by O'P'-DDT ranging from 2.2 μgKg-1 to 9.0 μgKg-1. Phylogenetic analysis proves that the Lactobacillus sakei Strain PRO7 bacteria was identified. The growth rate of DDT biodegradation by Lactobacillus sakei Strain PRO7 bacteria is noted the highest at 7.89 …