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To validate the effectiveness of a Physical Unclonable Function (PUF), it needs to be characterized over a large population of chips. Though simulation methods can provide approximate results, an on-chip experiment produces more accurate result. In this paper, we characterize a PUF based on ring oscillator (RO) using a significantly large population of 125 FPGAs. We analyze the experimental data using a ring oscillator loop delay model, and quantify the quality factors of a PUF such as uniqueness and reliability. The RO-PUF shows an average inter-die Hamming distance of 47.31%, and an average intra-die Hamming distance of 0.86% at normal operating condition. Additionally, we intend to make this large RO frequency dataset available publicly for the research community.