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Refined petroleum products may contain toxic compounds as additives causing environmental pollution, with adverse effects on living organisms. The study assessed the ecotoxicological effects and level of soil contamination of the tank farm area at a petroleum product depot in Ibadan, Nigeria. Surface soils (0-15 cm) and earthworms (Lumbricus terrestris) were sampled at four different tank areas; petroleum motor spirit (PMS), regular motor spirit (RMS), automotive gas oil (AGO) and dual purpose kerosene (DPK) as well as control site without prior petrochemical activities. Concentrations of heavy metals and total petroleum hydrocarbons (TPHs) were determined using atomic absorption spectrophotometer (AAS) and gas chromatography mass spectrophotometer (GC-MS). Earthworm’s lysosomal membrane stability, a biomarker was also determined using the neutral red retention time (NRRT) assay.