作者
Colin Dingwall, Ingemar Ernberg, Michael J Gait, Sheila M Green, Shaun Heaphy, Jonathan Karn, Anthony D Lowe, Mohinder Singh, Michael A Skinner, Robert Valerio
发表日期
1989/9
期刊
Proceedings of the National Academy of Sciences
卷号
86
期号
18
页码范围
6925-6929
简介
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.
引用总数
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C Dingwall, I Ernberg, MJ Gait, SM Green, S Heaphy… - Proceedings of the National Academy of Sciences, 1989