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In the present work, ceramic coatings are deposited on the surface of 2024-T3 aluminum (Al) alloy by micro-arc oxidation (MAO). The Surface roughness of the Al alloy substrate is different in the range from 0.2 μm to 1.6 μm. This paper investigates the effect of surface roughness of the Al alloy substrate and surface treatment on the fatigue properties of 2024-T3 Al alloy. The morphology, microstructure, and phase composition of the oxide layers are examined using X-ray diffraction (XRD) and scanning electron microscopy (SEM). Surface roughness of the coatings was measured using a 3D measuring laser microscope. The fatigue life of the Al alloy is evaluated under high and low cyclic stress. The results of the investigation have shown that the fatigue life of the coated samples with Ra = 0.2 μm is higher than others. The fatigue life of coated samples with Ra = 0.8 μm is very close to that with Ra = 1.6 μm …