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Enzyme-linked immunosorbent assay (ELISA)-, thin-layer chromatography (TLC)-, and highperformance liquid chromatography (HPLC)-sensitive methods were used for the identification of aflatoxin B1 (AFB1) contaminated fish feed, media, and fish serum samples.

The ELISA, TLC, and HPLC methods were validated by the quantitative and qualitative determination of AFB1 in contaminated fish feed, media, and fish blood serum samples.

The primary identification of AFB1 was carried out with a DOA-ELISA test kit ELISA followed by TLC with RF values 0.81, 0.79, 0.81, and 0.80 of AFB1-contaminated fish feed, media, and serum samples, respectively, compared with AFB1 standard. HPLC results show that the AFB1 levels in contaminated fish feed, media, and serum samples were 2.6, 2.6, and 2.7 ng/mL …