作者
Shubin Hong, Bo Lin, Minjie Xu, Quan Zhang, Zijun Huo, Mingyang Su, Chengcheng Ma, Jinyu Liang, Shuang Yu, Qiye He, Zhixi Su, Yanbing Li, Rui Liu, Zhuming Guo, Weiming Lv, Haipeng Xiao
发表日期
2023/4/1
期刊
EBioMedicine
卷号
90
出版商
Elsevier
简介
Background
Cell-free DNA (cfDNA) is being explored as biomarker for non-invasive diagnosis of cancer. We aimed to establish a cfDNA-based DNA methylation marker panel to differentially diagnose papillary thyroid carcinoma (PTC) from benign thyroid nodule (BTN).
Methods
220 PTC- and 188 BTN patients were enrolled. Methylation markers of PTC were identified from patients' tissue and plasma by reduced representation bisulfite sequencing and methylation haplotype analyses. They were combined with PTC markers from literatures and were tested on additional PTC and BTN samples to verify PTC-detecting ability using targeted methylation sequencing. Top markers were developed into ThyMet and were tested in 113 PTC and 88 BTN cases to train and validate a PTC-plasma classifier. Integration of ThyMet and thyroid ultrasonography was explored to improve accuracy.
Findings
From 859 potential PTC …
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S Hong, B Lin, M Xu, Q Zhang, Z Huo, M Su, C Ma… - EBioMedicine, 2023