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One of the important living organisms in stagnant waters, surface waters, water reservoirs, pools and dams is microalgae. Various methods have been developed and studied for the removal of these microscopic organisms from drinking water, of which the sedimentation or flocculation is one of the significant methods. The method is based on using sediments substance or flocculants. This study aimed to introduce feasible, harmless and suitable biological flocculants and evaluate their ability to precipitate Cyclotella diatoms. The presence of a large number of Cyclotella diatoms in water creates an unpleasant color and odor in the water. For this purpose, autoflocculation–sedimentation and bioflocculation processes were used to harvest Cyclotella. The effect of pH, mixing speed and time on harvest efficiency for autoflocculation–sedimentation and bioflocculation processes was investigated. The autoflocculation …