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Despite the high efficiency of microbial fuel cells (MFCs), MFCs cannot be a suitable alternative for treatment plants because of insufficient power generation and tiny reactors. Additionally, the increased reactor size and MFC stack result in a reduction in production power and reverse voltage. In this study, a larger MFC with a volume of 1.5 L has been designed called LMFC. A conventional MFC, called SMFC, with a volume of 0.157 L, was constructed and compared with LMFC. Moreover, the designed LMFC can be integrated with other treatment systems and generate significant electricity. In order to evaluate MFC's ability to integrate with other treatment systems, the LMFC reactor was converted into MFC-MBBR by adding sponge biocarriers. A 9.5 percent increase in reactor volume resulted in a 60 percent increase in power density from 290 (SMFC) to 530 (LMFC). An agitator effect was also investigated for better …