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This study was carried out for examination of a lab-scale UASB reactor for optimization of organic loading rate and hydraulic retention time in various operational temperatures. The total volume of the reactor was 5 l with an effective height of 160 cm and diameter of 5 cm. This reactor was used to treat fortified municipal wastewater at volumetric organic loadings of 3.6, 7.2, 10.8, and 14.4 kg m-3 d-1 at temperatures 30 °C and 20 °C and PH 7.6 – 8.4 for the treatment of high-strength enriched municipal wastewater of Ekbatan local treatment plant that located in west of Tehran. The COD of this wastewater have enriched by molasses and milk powder. To prevent PH fluctuation of the influent, NaHCO3 and K2HPO4 were added to wastewater. The result of present work indicated an optimum range for organic loading (7.2 to 10.8 kg m-3 d-1) at all temperature condition with COD removal efficiency of about of 85% and 73 …