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This study investigates the effect of MarT regulator protein on the production of Salmonella Typhimurium biofilm under in vitro culture conditions. The biofilm formation showed a statistically significant decrease compared to the wild-type strain at the two consecutive concentrations of arabinose (0.02% and 0.01%) in which the production of MarT protein was promoted to the highest level. When comparing the biofilm formation capacity to the wild type, the mutants containing a mutation in the genes investigated in terms of marT regulation showed statistically significant reductions, which were identified as fliA (59%), fliC (40%), fimA (46%), fidL (25%), and misL (25%) mutants. The biofilm structures of all strains used in this study were detected as the rdar morphotype in agar plates. Among the genes examined, fimA, slsA, fliA, dps, cheA, and wzzB genes were found to have a direct effect on the pellicle formation of S …