作者
Raymond T Suhandynata, Melissa A Hoffman, Deli Huang, Jenny T Tran, Michael J Kelner, Sharon L Reed, Ronald W McLawhon, James E Voss, David Nemazee, Robert L Fitzgerald
发表日期
2021/2/1
期刊
Clinical Chemistry
卷号
67
期号
2
页码范围
404-414
出版商
Oxford University Press
简介
Background
It is unknown whether a positive serology result correlates with protective immunity against SARS-CoV-2. There are also concerns regarding the low positive predictive value of SARS-CoV-2 serology tests, especially when testing populations with low disease prevalence.
Methods
A neutralization assay was validated in a set of PCR-confirmed positive specimens and in a negative cohort. In addition, 9530 specimens were screened using the Diazyme SARS-CoV-2 IgG serology assay and all positive results (N = 164 individuals) were reanalyzed using the neutralization assay, the Roche total immunoglobin assay, and the Abbott IgG assay. The relationship between the magnitude of a positive SARS-CoV-2 serology result and neutralizing activity was determined. Neutralizing antibody titers (50% inhibitory dilution, ID50) were also longitudinally monitored in …
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RT Suhandynata, MA Hoffman, D Huang, JT Tran… - Clinical Chemistry, 2021