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Provided are media and methods for establishing and maintaining mammalian early embryo-like cells. The culture media can be used to culture mammalian pluripotent stem cells (PSCs), are chemically defined, and comprise basal media for culturing stem cells supplemented with a S-adenosylhomocysteine hydrolase (SAH)/Polycomb repressive complexes (PRC)/EZH2 inhibitor and a histone deacetylase (HDAC) inhibitor. With the culture media thereof, primate (human and non-human) PSCs can be converted to preimplantation ICM-like cells (ICLCs) or 8-cell embryo-like cells (8CLCs).