查看文章

Glycine N-methyltransferase (GNMT) is known for its function as a tumor suppressor gene. Since 100% of female Gnmt^−/− mice developed hepatocellular carcinoma, we hypothesized that Gnmt^−/− mice may have defective immune surveillance. In this study, we examined the immune modulation of GNMT in T-cell responses using experimental autoimmune encephalomyelitis (EAE). The results showed that EAE severity was reduced significantly in Gnmt^−/− mice. Pathological examination of the spinal cords revealed that Gnmt^−/− mice had significantly lower levels of mononuclear cell infiltration and demyelination than the wild-type mice. In addition, quantitative real-time PCR showed that expression levels of proinflammatory cytokines, including interferon (IFN)-γ and interleukin (IL)-17A, were much lower in the spinal cord of Gnmt^−/− than in that of wild-type mice. Accordingly, myelin oligodendrocyte …