作者
Abdul Rehman Basharat, Kanzal Iman, Muhammad Farhan Khalid, Zohra Anwar, Rashid Hussain, Humnah Gohar Kabir, Maria Tahreem, Anam Shahid, Maheen Humayun, Hira Azmat Hayat, Muhammad Mustafa, Muhammad Ali Shoaib, Zakir Ullah, Shamshad Zarina, Sameer Ahmed, Emad Uddin, Sadia Hamera, Fayyaz Ahmad, Safee Ullah Chaudhary*
发表日期
2019/8/2
期刊
Scientific Reports
卷号
9
期号
11267
出版商
Nature Publishing Group
简介
Top-Down Proteomics (TDP) is an emerging proteomics protocol that involves identification, characterization, and quantitation of intact proteins using high-resolution mass spectrometry. TDP has an edge over other proteomics protocols in that it allows for: (i) accurate measurement of intact protein mass, (ii) high sequence coverage, and (iii) enhanced identification of post-translational modifications (PTMs). However, the complexity of TDP spectra poses a significant impediment to protein search and PTM characterization. Furthermore, limited software support is currently available in the form of search algorithms and pipelines. To address this need, we propose ‘SPECTRUM’, an open-architecture and open-source toolbox for TDP data analysis. Its salient features include: (i) MS2-based intact protein mass tuning, (ii) de novo peptide sequence tag analysis, (iii) propensity-driven PTM characterization, (iv) blind PTM …
学术搜索中的文章
AR Basharat, K Iman, MF Khalid, Z Anwar, R Hussain… - Scientific reports, 2019