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Hospital wastewater contains great amount of pathogenic organisms, hazardous chemicals, as well as organic and mineral materials, and one of the most important source of pollution with water resource is the hospital wastes which contain many pathogenic factors. Thus the necessity of sanitary collection, treatment and reuse of them is vital because of water shortage in recent decades, in which it requires the wastewater treatment plant establishing. For construction of such a treatment plant the quality and quantity parameters are needed. The most important quantity parameter is flow rate, which should be detected for determining of quality parameters. Since, many hospitals are not including wastewater treatment plant or if they are, the beneficence of their performance isn’t satisfactory. This research tried to investigate the pollution of special and upper special hospital in the biological oxidation method. The pilot was established in 2008 and after 2 months performance continuously, the treatment condition was proper and complied with environmental standard range in which the sampling was started in the late August and September. It is mentionable that at the end of every month three samplings were applied simultaneously and analyzed in order that the accuracy and veracity of the experiment become clarified. The results showed that all of the parameters were removable in the biological oxidation method. This procedure is usable for removal of physical, chemical and microbial parameters and heavy metals, and efficiency of biological oxidation removal method for the main hospital parameters was more than 90%.