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High frequency current and voltage measurements were used to determine passive electrical properties, such as the polarization effect at intact membrane interfaces and field-induced electropermeability changes in the cellular materials during direct current pulses. The time sequence of the electropermeabilization at the level of the cell membrane showed a similarity to the breakdown phenomena observed in cell systems (potato, apple and fish tissues, as well as plant cell suspension cultures) when a single pulse with critical or supercritical field amplitude is applied. A slight membrane breakdown phenomenon occurred in the first few microseconds after the initiation of the pulse at a critical electric field strength of 150–200 V/cm. Significant membrane breakdown was observed when the field strength of the electric pulses applied directly on the cell systems was in the range of 400–800 V/cm. At various field …