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Cell segmentation and/or localization is the first stage of a (semi)automatic tracking system. We addressed the cell localization problem in our previous work where we characterized a typical blood stem cell in a microscopic image as an approximately circular object with dark interior and bright boundary. We also addressed the modelling of adjacent and dividing cells in our previous work as a deconvolution method to model individual blood stem cell as well as adjacent and dividing blood stem cells where an optimization algorithm was combined with a template matching method to segment cell regions and locate the cell centers. Our previous cell deconvolution method is capable of modelling different cell types with changes in the model parameters. However in cases where either a complex parameterized shape is needed to model a specific cell type, or in place of cell center localization, an exact cell …