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Blastogenesis of human peripheral blood lymphocytes stimulated in vitro by non‐specific mitogens (PHA, ConA, PWM) upon exposure to extremely low frequency EMF has been studied. Different frequencies of square waveforms have been used. PHA‐stimulation resulted in strong inhibitions as measured by [³H]thymidine incorporation. A frequency window (3–50 Hz) within which ConA‐induced blastogenesis was significantly inhibited has been individuated. The mitogenic effect of PWM was significantly affected only at 3 Hz.