作者
Cheng Zhou, Jean-Michel Friedt, Angelina Angelova, Kang-Hoon Choi, Wim Laureyn, Filip Frederix, Laurent A Francis, Andrew Campitelli, Yves Engelborghs, Gustaaf Borghs
发表日期
2004/7/6
期刊
Langmuir
卷号
20
期号
14
页码范围
5870-5878
出版商
American Chemical Society
简介
Time-resolved adsorption behavior of a human immunoglobin G (hIgG) protein on a hydrophobized gold surface is investigated using multitechniques: quartz crystal microbalance/dissipation (QCM-D) technique; combined surface plasmon resonance (SPR) and Love mode surface acoustic wave (SAW) technique; combined QCM-D and atomic force microscopy (AFM) technique. The adsorbed hIgG forms interfacial structures varying in organization from a submonolayer to a multilayer. An “end-on” IgG orientation in the monolayer film, associated with the surface coverage results, does not corroborate with the effective protein thickness determined from SPR/SAW measurements. This inconsistence is interpreted by a deformation effect induced by conformation change. This conformation change is confirmed by QCM-D measurement. Combined SPR/SAW measurements suggest that the adsorbed protein barely …
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C Zhou, JM Friedt, A Angelova, KH Choi, W Laureyn… - Langmuir, 2004