作者
Greg Finak, Marc Langweiler, Maria Jaimes, Mehrnoush Malek, Jafar Taghiyar, Yael Korin, Khadir Raddassi, Lesley Devine, Gerlinde Obermoser, Marcin L Pekalski, Nikolas Pontikos, Alain Diaz, Susanne Heck, Federica Villanova, Nadia Terrazzini, Florian Kern, Yu Qian, Rick Stanton, Kui Wang, Aaron Brandes, John Ramey, Nima Aghaeepour, Tim Mosmann, Richard H Scheuermann, Elaine Reed, Karolina Palucka, Virginia Pascual, Bonnie B Blomberg, Frank Nestle, Robert B Nussenblatt, Ryan Remy Brinkman, Raphael Gottardo, Holden Maecker, J Philip McCoy
发表日期
2016/2/10
期刊
Scientific reports
卷号
6
期号
1
页码范围
1-11
出版商
Nature Publishing Group
简介
Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as pre-configured, lyophilized, reagents in 96-well plates. We present the results of a coordinated analysis of samples across nine laboratories using these panels with standardized operating procedures (SOPs). Manual gating was performed by each site and by a central site. Automated gating algorithms were developed and tested by the FlowCAP consortium. Centralized manual gating can reduce cross-center variability, and we sought to determine whether automated methods could streamline and standardize the analysis. Within-site variability was low in …
学术搜索中的文章
G Finak, M Langweiler, M Jaimes, M Malek, J Taghiyar… - Scientific reports, 2016