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Plastic pollution is now recognised as a growing concern for both the environment and associated biota. Consequently, a plethora of scientific reports are being produced regarding the occurrence, distribution, and transfer pathways of litter particles. However, there are scientific gaps that need to be filled regarding micro- and nanoplastic impacts on marine and freshwater organisms. This Special Issue (SI) aimed to provide an update on plastic pollution and its consequences on aquatic ecosystems, with a particular focus on biological domains, by adopting a multidisciplinary perspective. Several research articles and reviews were collected, focusing on the origin, fate, and effects of plastics in marine and freshwater environments, along with their interactions with biotic components. We believe this SI will contribute to the development of new studies on plastic pollution and the elaboration of science-based policies at …