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Security is important criteria for information transmission. This paper proposes highly secure method to hide the existence of secret message to prevent unauthorized access. The proposed method has three levels. Single level of encryption and two levels of steganography are used. First level makes use of DNA based RSA encryption. Second level hides the encrypted message inside the DNA sequence using randomized LSBase substitution technique. Third level hides the embedded DNA sequence inside the audio file using LSB encoding. The main objective of this method is that no one could be able to find the existence of secret message.