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This study investigates the chemical characteristics of rejuvenators and the effect of different rejuvenators on the morphology and fundamental behavior of rejuvenated asphalt binders. Gas chromatography-mass spectroscopy (GC-MS) and atomic force microscopy (AFM) were conducted to determine the chemical composition of rejuvenators and asphalt surfaces’ morphology. Also, surface free energy (SFE) measurements were performed to quantify the cohesive bond energy of rejuvenated binders. The thin film oven test (TFOT)-aged performance graded (PG) 58-28 binder was rejuvenated with waste cooking oil (UT), chemically modified waste cooking oil (TR), and Hydrolene H90T (HL) at concentrations of 3%, 6%, and 9% by weight of the total binder. To understand the compatibility, styrene-butadiene-styrene (SBS) was also blended with rejuvenated binders. The experimental study found the variability of free …