作者
Jinlei Yu, Yang Luo, Zhenlai Zhu, Yufeng Zhou, Licheng Sun, Jixin Gao, Jinlv Sun, Gang Wang, Xu Yao, Wei Li
发表日期
2019/6/1
期刊
Journal of Allergy and Clinical Immunology
卷号
143
期号
6
页码范围
2108-2119. e12
出版商
Mosby
简介
Background
Previous studies have revealed significant alterations in the skin microbiota of patients with atopic dermatitis (AD) not only in diversity and composition but also in function, and the tryptophan (Trp) metabolic pathway is attenuated in the skin microbiota of patients with AD.
Objective
We sought to assess Trp metabolites on the skin surfaces of patients with AD and to explore the function of the microbial Trp metabolites in skin inflammation in patients with AD.
Methods
A gel-patch method was developed to collect metabolites on the skin surface, which were then assessed by using liquid chromatography–tandem mass spectrometry. A mouse model of calcipotriol (MC903)–induced AD-like dermatitis was used to evaluate the effects of microbial metabolites on AD, and aryl hydrocarbon receptor (AhR)–null mice and keratinocyte cultures were used to investigate the mechanism.
Results
Major microbial …
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J Yu, Y Luo, Z Zhu, Y Zhou, L Sun, J Gao, J Sun… - Journal of Allergy and Clinical Immunology, 2019