作者
Licheng Sun, Jinrong Fu, Sheng-Hao Lin, Jin-Lyu Sun, Li Xia, Ching-Hsiung Lin, Lijuan Liu, Caiyan Zhang, Lan Yang, Ping Xue, Xiang Wang, Saihua Huang, Xiao Han, Hua-Ling Chen, Ming-Shyan Huang, Xiaobo Zhang, Shau-Ku Huang, Yufeng Zhou
发表日期
2020/1/1
期刊
Journal of Allergy and Clinical Immunology
卷号
145
期号
1
页码范围
402-414
出版商
Mosby
简介
Background
Epidemiologic evidence suggests that exposure to particulate matter of 2.5 μm or less in diameter (PM2.5) aggravates asthma.
Objective
We sought to investigate the underlying mechanisms between PM2.5 exposure and asthma severity.
Methods
The relationship between PM2.5 exposure and asthma severity was investigated in an asthma model with CD4+ T cell–specific aryl hydrocarbon receptor (AhR)–null mice. Effects of PM2.5 and polycyclic aromatic hydrocarbons (PAHs) on differentiation of TH17/regulatory T (Treg) cells were investigated by using flow cytometry and quantitative RT-PCR. Mechanisms were investigated by using mRNA sequencing, chromatin immunoprecipitation, bisulfite sequencing, and glycolysis rates.
Results
PM2.5 impaired differentiation of Treg cells, promoted differentiation of TH17 cells, and aggravated asthma in an AhR-dependent manner. PM2.5 and one of its prominent …
学术搜索中的文章
L Sun, J Fu, SH Lin, JL Sun, L Xia, CH Lin, L Liu… - Journal of Allergy and Clinical Immunology, 2020